منابع مشابه
Next-Generation Sequencing of Antibody Display Repertoires
In vitro selection technology has transformed the development of therapeutic monoclonal antibodies. Using methods such as phage, ribosome, and yeast display, high affinity binders can be selected from diverse repertoires. Here, we review strategies for the next-generation sequencing (NGS) of phage- and other antibody-display libraries, as well as NGS platforms and analysis tools. Moreover, we d...
متن کاملHigh-Throughput DNA Sequencing Analysis of Antibody Repertoires.
New high-throughput DNA sequencing (HTS) technologies developed in the past decade have begun to be applied to the study of the complex gene rearrangements that encode human antibodies. This article first reviews the genetic features of Ig loci and the HTS technologies that have been applied to human repertoire studies, then discusses key choices for experimental design and data analysis in the...
متن کاملBarcode-enabled sequencing of plasmablast antibody repertoires in rheumatoid arthritis.
OBJECTIVE A hallmark of rheumatoid arthritis (RA) is the production of autoantibodies, including anti-citrullinated protein antibodies (ACPAs). Nevertheless, the specific targets of these autoantibodies remain incompletely defined. During an immune response, B cells specific for the inciting antigen(s) are activated and differentiate into plasmablasts, which are released into the blood. We unde...
متن کاملThe Application of Next Generation Sequencing to the Understanding of Antibody Repertoires
In the decade since the human genome sequence was declared complete, the development of next generation sequencing (NGS) or "deep" sequencing to deliver cost-effective genomic sequencing has influenced advances beyond its primary application and changed the research landscape in many other areas. This review will survey recent applications of NGS which have broadened the understanding of natura...
متن کاملIdentification of functional anti-Staphylococcus aureus antibodies by sequencing patient plasmablast antibody repertoires
Please cite this article as: D.R. Lu, et a antibody repertoires..., Clin. Immuno R E C
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ژورنال
عنوان ژورنال: mAbs
سال: 2011
ISSN: 1942-0862,1942-0870
DOI: 10.4161/mabs.3.1.14169